Invitrogen™ Dynabeads™ Carboxylic Acid for RNA Purification

Dynabeads Carboxylic Acid for RNA Purification are monosized (1 μm), monodispersed, paramagnetic beads characterized by high speed to magnet and high pellet stability. They are intended for use with Dynabeads RNA Binding Buffer, for simple, scalable, and automation-ready purification of crude RNA produced by in vitro transcription (IVT). The combination is particularly suitable where flexibility and high purity are required, such as during the discovery phase of mRNA vaccine development and following the solid-phase IVT reaction with Dynabeads Streptavidin for In Vitro Transcription.

Features of Dynabeads Carboxylic Acid for RNA Purification include:

• Efficiency—reuse at least six times to reduce the cost and time in a simple workflow
• Scalability—swap between manual and automated workflows to match your required scale (micrograms to grams of mRNA) and throughput
• Quality—ensure safe and flexible supply of magnetic beads to navigate from RNA-based drug discovery to commercial manufacturing without incurring extra time or costs from switching technology or raw material

Crude RNA from an IVT reaction step is mixed with the beads and the buffer is added. The RNA binds to the bead surface and the remaining components of the reaction mixture are removed by applying a magnet and discarding the supernatant.

The bead concentration is 10 mg/mL. They are stored in purified water and may be recycled at least six times. Scripts are available for KingFisher automated purification workflows.

The beads are offered here as a stand-alone product. The are also included in the kits below:

• Dynabeads RNA Purification Kit (Cat. No. 65030D)
• Dynabeads In Vitro Transcription and RNA Purification Kit (Cat. No. 65034D)

To learn more about other products and support documentation available for GMP-compliant manufacturing of mRNA vaccines and therapeutics visit: thermofisher.com/dynabeadsmrna