Thermo Scientific™ DyLight™ Orange Specialty Dyes

Highlights:

• NHS ester reactive group–allows immediate labeling of antibodies, proteins, peptides and other amine-containing molecules through amide bond formation

• Multiple solubility options –choose from hydrophilic to hydrophobic dyes to optimize the right dye label for the best performance in a given application

Criteria to consider when choosing a DyLight Orange-emitting Specialty Dye:

• Excitation and emission wavelengths –choose the best dye to match the excitation and emission capabilities of your instrument
• Water solubility–choose a dye based on its relative hydrophilicity, which directly correlates to the number of negatively-charged sulfonates it has on its core structure. More hydrophilic dyes are best at maintaining water solubility of a labeled antibody and limiting the nonspecific binding of the conjugate. More hydrophobic dyes often are best at penetrating tissues and cell membranes in vivo, meaning that dyes with fewer sulfonates may work best for some applications.

Recommended for:

Imaging; Antibody labeling; Direct immunofluorescence staining; Flow cytometry; Fluorescence correlation spectroscopy; ELISA; Western blotting; Protein microarrays; Polymer labeling; Peptide labeling; Phalloidin labeling for actin staining; Staining in acidic media; Biofilm microorganism staining