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  5. Applied Biosystems™ Custom TaqMan™ SNP Genotyping Assays

Applied Biosystems™ Custom TaqMan™ SNP Genotyping Assays

Código de producto. 32226596 Tienda Applied Biosystems Productos
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Tipo de muestra:
Human or Non-human
No humano
Cantidad:
1000 Reactions, Made to Order
2400 Reactions, Made to Order
300 Reactions, Made to Order
L (2400 reactions), Made to Order
M (1000 reactions), Made to Order
S (300 reactions), Made to Order
Tamaño de la unidad:
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paquete de 40
paquete de 80
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Código de producto. Tipo de muestra Cantidad Tamaño de la unidad
32226596 Human or Non-human 2400 Reactions, Made to Order cada uno
13435096 No humano S (300 reactions), Made to Order cada uno
15531034 No humano M (1000 reactions), Made to Order paquete de 40
15541034 No humano L (2400 reactions), Made to Order paquete de 80
32226515 Human or Non-human 300 Reactions, Made to Order cada uno
32226521 Human or Non-human 1000 Reactions, Made to Order cada uno
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Código de producto. 32226596 Proveedor Applied Biosystems™ N.º de proveedor A80739
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Fecha estimada de envoi: 13-01-2027
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Descripción

Provide a highly flexible technology for detection of polymorphisms within any genome.

Applied Biosystems TaqMan SNP Genotyping Assays use TaqMan 5'-nuclease chemistry to amplify and detect specific polymorphisms in purified genomic DNA samples. Each assay enables genotyping of individuals for a single nucleotide polymorphism (SNP) and consists of two sequence-specific primers and two TaqMan minor groove binder (MGB) probes with non-fluorescent quenchers (NFQ). Assays contain one probe labeled with VIC or Cyanine 5 dye to detect the Allele 1 sequence; the second probe is labeled with FAM or ABY dye to detect the Allele 2 sequence.

Custom TaqMan SNP Genotyping Assays can be easily designed at no extra charge by submitting target sequences confidentially to our secure Custom Assay Design Tool.

Benefits:

  • Proven—gold-standard TaqMan chemistry and robust assay designs deliver accurate, reproducible, and reliable results
  • Easy—convenient single-tube format and simple workflow provide an easy path to trusted results; no optimization required
  • Flexible—obtain assay designs for any SNP in the human genome using our secure Custom Assay Design Tool, at no extra charge
  • Tested—all custom human assays (VIC/FAM SKUs only) are subjected to a functional test on 20 unique DNA samples. Assays ordered with non-human or Cyanine 5/ABY SKUs are not functionally tested.
  • Multiplexing ready—TaqMan assays are now available with with VIC/FAM or Cyanine 5/ABY formats to support simultaneous detection of two sets of SNPs (two pairs of alleles).

Approximate ship time

4–6 days in North America and 6–10 days in Europe (VIC/FAM); 4-10 days in North America, 6-14 days in Europe (Cyanine 5/ABY)

The free Custom Assay Design Tool can generate assays for the detection of SNPs, MNPs, and indels of up to six bases. These custom assays are designed, synthesized, formulated, optimized, and quality control tested.

TaqMan SNP Genotyping Assays require only three reaction components for PCR: purified genomic DNA (1–20 ng), the assay solution, and TaqMan Genotyping Master Mix (singleplex) or TaqPath ProAmp Master Mix (multiplex) (sold separately).

All assay designs are the product of our industry-leading bioinformatics pipeline, optimized over the course of more than a decade by leveraging manufacturing and assay performance data. TaqMan Assays have been cited in over 40,000 publications and are backed by more than 350 patents.

Recommended master mix (sold separately): TaqMan Genotyping Master Mix (Cat. No. 4371355) or TaqPath ProAmp Master Mix (Cat. No. A30865)

TRUSTED_SUSTAINABILITY

Especificaciones

Concentración 80X
Contenido y almacenamiento •1 tube containing a 40X (S and M sizes) or 80X (L size) mix of pre-formulated assay (2 probes and 2 primers).
•Store at -15°C to -25°C.
Para utilizar con (aplicación) Genotyping
Para utilizar con (equipo) 7500 System, 7500 Fast System, 7900HT System, StepOne, StepOnePlus, ViiA 7 System, QuantStudio Absolute Q Digital PCR System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 12k Flex ProFlex PCR System*, VeritiPro*, SimpliAmp*, MiniAmp*, Automated Thermal Cycler** If a thermal cycler is used for PCR amplification, the optional pre-read and the post-read must be performed separately on a real-time PCR system in order to detect and record fluorescent signals.
de control principal Cyanine 5 - MGB probe detects Allele 1, ABY - MGB probe detects Allele 2
N.º de reacciones 2400 Reactions, Made to Order
Línea de productos TaqMan
Tipo de producto SNP Genotyping
Cantidad 2400 Reactions, Made to Order
Condiciones de envío Room Temperature
Formato Tube
Tipo de muestra Human or Non-human
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Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

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