Applied Biosystems™ Custom TaqMan™ Small RNA Assay

Custom TaqMan Small RNA Assays use TaqMan Real-Time PCR Assays for the analysis of any small RNA molecule. The novel adaptations in TaqMan Assay design developed for the study of miRNAs are ideal for analysis of any small nucleic acid less than 200 bases long, including newly discovered miRNAs, Piwi-interacting RNA (piRNA), small nuclear RNA (snRNA), and small nucleolar RNA (snoRNA).

• Flexible and convenient ordering—easy process with our secure Custom TaqMan Small RNA Assay design tool
• Versatile application—use for the analysis of any small RNA from any species
• Reliable outcome—pre-formulated assays are optimized to run under universal conditions
• Powerful miRNA quantitation—the same sensitivity, specificity, and reproducibility as predesigned TaqMan MicroRNA Assays

Sophisticated design pipeline
The miRPipe Small RNA Assay design pipeline is founded on our extensive background and bioinformatics expertise designing TaqMan Gene Expression Assays. To detect very small targets, it includes design features based on empirical data gathered over years of experiments. The resulting automated pipeline includes built-in flexibility features enabling design of assays for the broadest assortment of small RNA sequences. The benefits of TaqMan Assay technology are ideal for analysis of virtually any small RNA, enabling results you can trust from the very first experiment.

Pre-formulated assay
• Forward primer: 1500 nM
• Reverse primer: 700 nM
• FAM-MGB probe: 200 nM
• RT sequences: 50 nM

Ordering information
First, bioinformatically qualify your sequence target for specificity and sequence quality, then use the Custom TaqMan Small RNA Assay Design Tool to input your sequence and submit it for assay design. This easy-to-use tool is a secure and confidential. If we are unable to design or manufacture an assay for your sequence target of interest, you will not be charged.