BD CompBead Anti-Mouse Ig, κ/Negative Control Particles Set

The set provides two populations of microparticles:

• BD™ CompBeads Anti-Mouse Ig, κ—Binds any mouse κ light chain-bearing immunoglobulin
• BD™ CompBeads Negative Control—Has no binding capacity

When mixed together with a fluorochrome-conjugated mouse antibody, the CompBeads provide distinct positive and negative (background fluorescence) stained populations which can be used to set compensation levels manually or using instrument set-up software.

Since the compensation adjustments are made using the same fluorochrome-labeled antibody to be used in the experiment, this method allows the investigator to more accurately establish compensation corrections for spectral overlap for any combination of fluorochrome-labeled antibodies (without having to use valuable tissue samples or hard-dyed beads with potentially mismatched fluorescence spectra).

Use of the BD™ CompBeads is highly recommended for use in all experiments using tandem dye (i.e., PE-Cy™7, APC-Cy™7, etc.) conjugates, which may have distinct spectral characteristics for each conjugate.