Invitrogen™ CD45 Monoclonal Antibody (HI30), Brilliant Ultra Violet™ 395, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes. Applications Reported: This HI30 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HI30 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Brilliant Ultraviolet™ 395 (BUV395) is a dye that emits at 395 nm and is intended for use on cytometers equipped with an ultraviolet (355 nm) laser. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright, Brilliant Violet™, Brilliant Ultraviolet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize any non-specific polymer interactions. Excitation: 350 nm; Emission: 395 nm; Laser: Ultraviolet Laser.