CD223 (LAG-3) Monoclonal Antibody (3DS223H), NovaFluor™ Yellow 700, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

This 3DS223H mAb recognizes human CD223 also known as Lymphocyte Activation Gene 3 (LAG-3). LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells). This 3DS223H antibody will recognize a formaldehyde-fixed epitope. Applications Tested: This 3DS223H antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5uL (0.06ug) per test Our internal testing shows that NovaFluor Yellow 700 non-specifically stains B cells in SJL mice. Non-specific staining has not been observed in BALB/c or C57BL/6 mice. See the Antibody Testing Data for an example of this strain-dependent difference. NovaFluor dyes are not compatible with DNA intercalating viability dyes. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes. Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer. Excitation: 552nm; Emission: 700nm; Laser: 561nm (Yellow) Laser