Thermo Scientific™ CaptureSelect™ IgG-Fc (rabbit) Affinity Matrix

Features of this affinity matrix include:

• Highly selective for rabbit IgG
• No binding to mouse, rat, human, bovine, horse, sheep, and goat IgG
• Complex growth media with fetal calf or horse serum can be applied
• Non-animal-derived

The high selectivity and yields obtained using CaptureSelect IgG-Fc (Rabbit) Affinity Matrix enables a robust and efficient purification process with excellent purity obtained in one step.

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics:

• Matrix: agarose-based
• Average particle size: 65 ± 10 μm
• Ligand: CaptureSelect IgG-Fc (rabbit) affinity ligand
• Ligand coupling method: epoxide
• Binding capacity: >15 mg rabbit IgG/mL resin
• Elution conditions: 1 M glycine, pH 3.0, or 20 mM acetic or citric acid, pH 3.0
• Flow characteristics: 50–200 cm/h (up to 2 bar)
• Formulation buffer: 20%(v/v) ethanol