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Descripción
CaptureSelect IgE Affinity Matrix has been designed specifically for the purification of human IgE (immunoglobuline E) from recombinant and plasma sources.
Features of this affinity matrix include:
- Purification of human IgE, binding different recombinant IgE forms
- Efficient removal of host cell proteins (HCP) and IgE aggregates
- Excellent scalability
- Non–animal-derived
Free of animal components
CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).
Main characteristics
- Matrix: agarose-based, epoxide-activated
- Average particle size: 65 ± 10 μm
- Ligand: CaptureSelect IgE affinity ligand
- Ligand coupling method: aldehyde coupling
- Binding capacity: >5 mg/mL resin
- Elution conditions: 50 mM sodium citrate, 150 mM NaCl, pH 3.5
- Flow characteristics: 50–200 cm/h (up to 2 bar)
- Formulation buffer: 20% (v/v) ethanol
Especificaciones
Especificaciones
| Capacidad de unión | >5 mg/mL resin |
| Caudal | 50 to 200 cm/hr. (Up to 2 bar) |
| Matriz | Agarose-based, epoxide-activated |
| Contenido y almacenamiento | Store at 2°C to 8°C. |
| Condiciones de envío | Temperatura ambiente |
| Tamaño de partículas | 65 ±10 μm |
| Formulario | Chromatography Media |
| Línea de productos | CaptureSelect |
| Cantidad | 5 mL |
| Tipo | Affinity Matrix |
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Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
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