Thermo Scientific™ CaptureSelect™ Human Growth Hormone Affinity Matrix

Features of this affinity matrix include:

• Highly selective for recombinant hGH
• Efficient purification of recombinant hGH with high yields and retained activity
• Excellent scalability
• Non-animal-derived

Recombinant hGH, also known as somatotropin or somatropin, is a peptide hormone that stimulates growth, cell reproduction, and regeneration in humans and other animals. The high selectivity and yields obtained using CaptureSelect Human Growth Hormone Affinity Matrix enable a robust and efficient purification process with excellent purity obtained in one step.

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics:

• Matrix: agarose-based, aldehyde-activated
• Average particle size: 65 ± 10 μm
• Ligand: CaptureSelect hGH affinity ligand
• Ligand coupling method: aldehyde coupling via NH₂ residues of the ligand
• Binding capacity: ∼3 mg/mL resin
• Elution conditions: 20 mM citric acid, pH 3.0
• Flow characteristics: 50–200 cm/h (up to 2 bar)
• Formulation buffer: 20%(v/v) ethanol