Thermo Scientific™ CaptureSelect™ FcXP Pre-packed Column

CaptureSelect FcXP Affinity Matrix is the successor to CaptureSelect FcXL Affinity Matrix, specifically designed for higher binding capacity. The selectivity is identical; the ligand recognizes the CH3 domain of all human IgG subclasses (IgG1, 2, 3, and 4). But the matrix features an increased binding capacity (>40 g IgG/L), while maintaining the elution characteristics of CaptureSelect FcXL Affinity Matrix.

Features of this affinity matrix include:

• Mild elution, making it suitable for Fc fusion proteins
• Human specific, no binding to bovine antibodies
• Excellent scalability
• Non-animal derived

CaptureSelect FcXP pre-packed columns can be used for the purification of IgG and Fc fusion proteins directly from complex source materials and enable a robust and efficient purification process with excellent purity in one step.

Characteristics:

• Matrix: agarose-based, epoxide activated
• Average particle size: 65 ± 10 μm
• Ligand: CaptureSelect FcXP affinity ligand
• Ligand coupling method: epoxide coupling
• Binding capacity: 40–50 g IgG/L resin depending on flow rate, column height, and contact time
• Elution conditions: 20 mM citric acid or acetic acid, pH 3–4, or 100 mM Tris, 2.0 M MgCl₂, 40% (v/v) propylene glycol, pH 7
• Flow characteristics: 150–300 cm/h (up to 2 bar)
• Formulation buffer: 20% (v/v) ethanol