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  6. Thermo Scientific™ CaptureSelect™ CH1-XL Magnetic Agarose Beads

Thermo Scientific™ CaptureSelect™ CH1-XL Magnetic Agarose Beads

Código de producto. 30275933 Tienda Thermo Scientific Productos
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Código de producto. 30275933 Proveedor Thermo Scientific™ N.º de proveedor 2883462001

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Descripción

Thermo Scientific™ CaptureSelect™ CH1-XL Magnetic Agarose Beads are high-capacity, high-throughput magnetic affinity particles for antibody purification using manual or robotic magnetic separators.

CaptureSelect CH1-XL Magnetic Agarose Beads are high-capacity, high-throughput magnetic affinity particles for antibody purification using manual or robotic magnetic separators. The CaptureSelect CH1-XL affinity ligand recognizes the CH1 domain of human IgG, enabling purification of all subclasses (1, 2, 3, and 4) of human IgG and Fab fragments, independent of the light-chain isotype and source material. Due to selectivity for the CH1 domain, no co-purification of free light chains will occur. IgG and Fab fragments can be directly isolated from complex source materials in a single step with high purity and yield.

Features of CaptureSelect CH1-XL Magnetic Agarose Beads include:

  • One-step human IgG purification with high purity, from crude material
  • Compatible with manual and automated workflows (e.g., Thermo Scientific KingFisher instruments)
  • Short operation time
  • Mild elution at pH 4
  • No sample dilution required during isolation, repetitive sample loading feasible
  • No binding to bovine antibodies, allowing use of fetal bovine serum in the sample material
  • Non-animal-derived

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

Main characteristics:

  • Matrix: 6% cross-linked magnetized agarose
  • Average particle size: 25–30 μm
  • Suspension: 25% slurry
  • Ligand: CaptureSelect CH1-XL affinity ligand
  • Binding capacity: >4 mg IgG/mL slurry
  • Elution conditions: 50 mM sodium acetate, pH 4.0/0.1 M glycine, pH 3.0

Especificaciones

Capacidad de unión >4 mg IgG/mL slurry
Matriz 6% cross-linked magnetized agarose
Tamaño de partículas 25 to 30 μm
Formulario Pulpa de resina
Línea de productos CaptureSelect
Cantidad 1 mL
Tipo Magnetic Agarose Bead
Tipo de ligando CH1-XL affinity ligand
Título del producto
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