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Invitrogen™ BrdU Monoclonal Antibody (BU-1)

Código de producto. 10009169
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Cantidad:
100 μL
Tamaño de la unidad:
100 microlitros
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10009169 100 μL 100 microlitros
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Código de producto. 10009169 Proveedor Invitrogen™ N.º de proveedor MA3071
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Mouse Monoclonal Antibody

MA3-071 detects 5-Bromo-2-deoxyuridine (BrdU) in many samples. MA3-071 has been successfully used in immunofluorescence, immunohistochemistry, and flow cytometry applications. The MA3-071 immunogen is 5-iodouridine covalently coupled to ovalbumin. This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues, and can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
TRUSTED_SUSTAINABILITY

Especificaciones

Antígeno BrdU
Aplicaciones Flow Cytometry, Immunohistochemistry, Immunocytochemistry
Clasificación Monoclonal
Clon BU-1
Concentración 0.05 mg/mL
Conjugado Unconjugated
Formulación 0.01M HEPES with 0.15M NaCl, 30% glycerol and 0.01% sodium azide
Alias de gen 5-Bromo-2-deoxyuridine; Bromodeoxyuridine
Especie del huésped Mouse
Inmunógeno 5-iodouridine covalently coupled to ovalbumin.
Método de purificación Protein A
Cantidad 100 μL
Estado normativo RUO
Primario o secundario Primary
Especies diana Chemical
Contenido y almacenamiento -20°C
Tipo de producto Antibody
Formulario Liquid
Isotype IgG2a
Molécula diana Chemical
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