CD3 Mouse anti-Human, BD IMag-DM, Clone: HIT3A, BD

BD IMag™ Anti-Human CD3 Particles DM are magnetic nanoparticles that have monoclonal antibody conjugated to their surfaces. These particles are optimized for the positive selection or depletion of CD3-bearing leukocytes using the BD IMagnet™. CD3 has been reported to be expressed on all mature T lymphocytes and on developing thymocytes.

Peripheral Blood Mononuclear Cells (PBMC) are labeled with BD IMag™ anti-human CD3 Particles - DM according to the Magnetic Labeling Protocol. This labeled cell suspension is then placed within the magnetic field of the BD IMagnet™ (Cat. No. 552311). Labeled cells migrate toward the magnet (positive fraction), leaving the unlabeled cells in suspension so they can be drawn off (negative fraction). The tube is then removed from the magnetic field for resuspension of the positive fraction. The separation is repeated to increase the purity of the positive fraction. The magnetic separation steps are diagrammed in the Separation Flow Chart. After the positive fraction is washed, the small size of the magnetic particles allows the positive fraction to be further evaluated in downstream applications such as flow cytometry.