alpha-1 Antitrypsin Mouse anti-Human, Clone: 3B7, Invitrogen™

Mouse Monoclonal Antibody

HYB 191-01-02 has specificity for human a-1 AT, native and denatured.   HYB 191-01-02 has been successfully used in Western blotting and ELISA procedures. HYB 191-01-02 reacts strongly with a-1 AT. Strong reaction is seen in ELISA with native and denatured a-1 AT directly coated onto the microtiter well. When tested in sandwich ELISA in combination with a polyclonal antibody against a-1 AT (e.g. DAKO A0012), it only recognizes native a-1 AT. In Western blotting after SDS-PAGE, HYB 191-01-02 reacts with native and denatured a-1 AT in reduced as well as non-reduced forms.  The HYB 191-01-02 immunogen is a-1 AT isolated from human plasma. The HYB 191-01-02 epitope differs from that of HYB 185-02-02. NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL