Applied Biosystems™ AccuSEQ™ Real-Time PCR Detection Software v3.2

AccuSEQ Real-Time PCR Detection Software v3.2 is part of an integrated workflow for impurity and contaminant testing during biopharmaceutical manufacturing using the SEQ family of real-time PCR assays, including MycoSEQ, resDNASEQ, and ProteinSEQ assays. The software is designed to work with the Applied Biosystems QuantStudio 5 Real-Time PCR System with 0.1-mL, 96-well plates for either Fast or Standard cycling. This software is provided via a link for online download.

Features of AccuSEQ Real-Time PCR Detection Software include:
• Accurate host cell impurities quantitation for resDNASEQ Host Cell Residual DNA Quantitation assays
• Automated presence/absence calls for MycoSEQ Mycoplasma and ViralSEQ MMV/Vesivirus assays
• Accurate quantitation of Sf-rhabdovirus genomic RNA for ViralSEQ Quantitative Sf-Rhabdovirus Kit
• Single software platform for all current and future SEQ real-time PCR assays
• Security, audit, and e-signature capabilities to help enable 21 CFR Pt 11 compliance

Residual DNA quantitation
For host cell residual DNA quantitation, a custom module with next-generation algorithms can be used with the resDNASEQ CHO Residual DNA Assay for accurate quantitation.

Mycoplasma detection
For rapid and accurate Mycoplasma detection, AccuSEQ software offers a plug-in analysis module for automated presence/absence calls when using the MycoSEQ Mycoplasma Detection system. Automated analysis tools enable one-click processing of MycoSEQ assay data, helping deliver presence or absence calls within seconds of data collection being completed.

MMV and vesivirus detection
AccuSEQ software is optimized and validated for the ViralSEQ MMV and Vesivirus Detection assay. The software’s visual result summary provides immediate understanding of presence or absence of virus in your samples.

Sf-rhabdovirus genomic RNA quantitation
AccuSEQ software helps enable rapid and easy analysis of ViralSEQ Quantitative Sf-Rhabdovirus system data without the hassle of multiple exporting steps and reliance on third party software.