missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Enzymes and Inhibitors
  4. Restriction Enzymes
  5. Thermo Scientific™ BspLI (NlaIV) (10 U/μL)

Thermo Scientific™ BspLI (NlaIV) (10 U/μL)

Product Code. 10314720 Shop All Thermo Scientific Products
Change view
Click to view available options
Quantity:
1000 U
200 units
Unit Size:
1000 units
200 units
Product Code. Quantity unitSize
10314720 1000 U 1000 units
10898990 200 units 200 units
2 options
This item is not returnable. View return policy

Product Code. 10314720

Brand: Thermo Scientific™ ER1152

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Cut at GGN^NCC sites with BspLI (NlaIV) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BmiI, NlaIV, PspN4I).

Conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'..G G N▵N C C...3'

3'..C C N▵N G G...5'

Conditions for 100% Activity:

  • 1X Buffer Tango: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • BspLI is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 200mM NaCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with BspLI, approximately 90% of the DNA fragments can be ligated and more than 95% of these can be recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: may overlap — cleavage impaired
  • CpG: may overlap — cleavage impaired
  • EcoKI: never overlaps — no effect
  • EcoBI: never overlaps — no effect

Note:

BspLI cleavage is impaired by overlappingdcm methylation. To avoiddcm methylation, use adam-, dcm strain such as GM2163 (#M0099).

TRUSTED_SUSTAINABILITY

Specifications

Concentration 10 U/μL
Incubator Temperature 37°C
Quantity 1000 U
Product Type BspLI (NlaIV)

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.