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ELISA kits are ideal for the quantification of target antigens in tissue extracts, serum and cell culture.

Rabbit Polyclonal Antibody

Alias de gen	53BP1, p202, p53-binding protein 1, p53bp1, TDRD30, TP53-Binding Protein 1, tumor protein 53-binding protein, 1, tumor protein p53 binding protein 1, tumor protein p53-binding protein, 1, tumor suppressor p53-binding protein 1
Símbolos de los genes	TP53BP1

Mouse Monoclonal Antibody

Primario o secundario	Primary
Formulación	0.2um-filtered solution in PBS, pH 7.4.
Método de purificación	Protein G purified
Concentración	1 mg/mL
Inmunógeno	Recombinant Listeria monocytogenes p60.
Dilución	Western Blot, ELISA
Formulario	Purified
Clon	p6007
Isotype	IgG1 κ
Especie del huésped	Mouse
Conjugado	Unconjugated
Antígeno	Listeria monocytogenes p60
Aplicaciones	Western Blot,ELISA
Contenido y almacenamiento	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Alias de gen	IAP p60 from Listeria Monocytogenes, Invasion-associated Protein p60, Invasion-associated Protein p60 from Listeria Monocytogenes, invasion-associated-protein, p60 protein
Especificidad de la prueba	Recognizes Listeria monocytogenes p60 protein. Does not cross-react with other Listeria species.
Especies diana	Bacteria
Estado normativo	RUO
Clasificación	Monoclonal

Native Protein

Rabbit Polyclonal Antibody has been used in 36 publications

Applications: Functional

Assay Kit (Colorimetric)

Mouse Monoclonal Antibody has been used in 6 publications

Primario o secundario	Primary
Método de purificación	Protein G purified
Concentración	1 mg/mL
Peso molecular del antígeno	15 kDa
Inmunógeno	This Histone H2AX [p Ser139] Antibody (3F2) was developed against a synthetic peptide sequence surrounding phosphorylated Ser139.
Dilución	Western Blot 1 μg/mL, Simple Western 10 μg/mL, Flow Cytometry 1 μg 10⁶ cells, ELISA 1:100 - 1:2000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 2 - 4 μg/mL, Immunohistochemistry-Paraffin 1:10 - 1:500
Formulario	Purified
Clon	3F2
Isotype	IgG1 κ
Símbolos de los genes	H2AFX
Especie del huésped	Mouse
Conjugado	Unconjugated
ID de gen (Entrez)	3014
Antígeno	Histone H2AX (p Ser139)
N.º de referencia del gen	P16104
Disciplina de investigación	Checkpoint signaling, DNA Double Strand Break Repair, DNA Repair, Epigenetics, Mitotic Regulators, Phospho Specific
Aplicaciones	Western Blot
Contenido y almacenamiento	Store at -20°C. Avoid freeze/thaw cycles.
Alias de gen	H2A.X, H2A/X, H2AFX
Especificidad de la prueba	In Western blot this antibody detects ∼17 kDa protein representing phosphorylated H2AX in gamma irradiated HeLa cell lysate. In immunofluorescence procedures, recognizes phosphorylated H2AX in gamma irradiated HeLa cells. ELISA of phosphorylated H2AX can also be performed. Used in IHC to successfully detect H2A.X pSer140 in postnatal mouse lung section.
Especies diana	Human
Estado normativo	RUO
Clasificación	Monoclonal

Recombinant Protein

Requisitos de almacenamiento	Store at -20°C to -70°C as supplied. After reconstitution, store at 2°C to 8°C for 1 month and at -20°C to -70°C for long term storage. Avoid repeated freeze-thaw cycles.
Formulación	Lyophilized from additive free solution.
Concentración	LYOPH
Reconstitución	Dissolve in distilled water or saline.
Expresión	Protein A
Alias de gen	Immunoglobulin G binding protein A, SPA, Staphylococcal protein A
ID de gen (Entrez)	3919448
Categoría de investigación	Epitope Tags
Concentración de endotoxinas	Less than 0.1 EU/ug of Protein A as determined by LAL method.
Grado de pureza o calidad	>97% pure by SDS-PAGE and HPLC
Peso molecular	33.4 kDa
Para utilizar con (aplicación)	PAGE,Bioactivity,HPLC