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Invitrogen™ PDGF-BB Porcine ELISA Kit
Sandwich ELISA Kit
672.00€ - 2640.00€
Especificaciones
Número de acceso | P20034 |
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Sensibilidad del ensayo | 10 pg/ml |
ensayo | ELISA sandwich de fase sólida |
Conjugado | Biotina |
Tipo de producto | Kit de ELISA |
Código de producto | Marca | Cantidad | Precio | Cantidad y disponibilidad | |||||
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Código de producto | Marca | Cantidad | Precio | Cantidad y disponibilidad | |||||
15471124
|
Invitrogen™
ESPDGFB |
96 pruebas |
672.00€
1 set |
Fecha estimada de envío: 21-05-2024
Inicie sesión para ver el stock |
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17155979
|
Invitrogen™
ESPDGFBX5 |
5 x 96 pruebas |
2640.00€
cada uno |
Fecha estimada de envío: 28-05-2024
Inicie sesión para ver el stock |
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Descripción
The Porcine Platelet-Derived Growth Factor BB (Porc PDGF BB) ELISA quantitates Porc PDGF BB in porcine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Porc PDGF BB. Principle of the method The Porcine PDGF BB solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor alpha polypeptide , where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene.Especificaciones
P20034 | |
ELISA sandwich de fase sólida | |
Kit de ELISA | |
Plasma, Sobrenadante, Suero | |
96 ensayos | |
Lector de microplacas colorimétrico | |
PDGF-2 | |
< 10 % | |
Peroxidasa de rábano picante (HRP) | |
Ciclo y proliferación celular | |
De 2 °C a 8 °C | |
1 h 20 min |
10 pg/ml | |
Biotina | |
Receptores y factores de crecimiento | |
Este par de anticuerpos ELISA detecta PDGF-BB porcino y humano. Otras especies no determinadas. | |
ELISA, ensayos de proteínas, biología de proteínas, biología del cáncer, inmunología, neurociencia, neurología, detección de proteínas, análisis de proteínas | |
100626465 | |
< 12 % | |
Placa de 96 pocillos previamente recubiertos, patrón, concentrado de diluyente de muestra, anticuerpo de detección biotinilado, SAV-HRP, tampón de lavado, cromógeno, solución de parada, cubiertas de placa adhesivas | |
RUO | |
50 μl de plasma; 50 μl de suero; 100 μl de sobrenadante | |
Porcino | |
4 h 45 min |