Invitrogen™ ToxBLAzer™ DualScreen Kit
ToxBLAzer™ DualScreen Kit
Marca: Invitrogen™ K1139
Detalles adicionales : Peso : 1.77000kg
DescripciónToxBLAzer™ DualScreen Kit provide the key advantage of combining the ratiometric reporter gene readout of beta-lactamase with a cytotoxicity readout in the same assay. Thus, ToxBLAzer™ DualScreen yields reporter readouts that normalize cytotoxic cellular responses, which is a necessity in screening applications which down regulate transcription. The ToxBLAzer™ substrate contains both the LiveBLAzer™-FRET B/G substrate and a proprietary cytotoxicity indicator. Both compounds readily enter the cell, where endogenous esterases rapidly convert them into fluorescent compounds Esterase activity and an intact cell membrane are required for detection of the indicator. The cytotoxicity indicator's fluorescence is red-shifted to ensure compatibility with the blue and green emissions of the LiveBLAzer™-FRET B/G substrate, allowing detection of both reporter gene activity and cytotoxicity in the same assay sample.
Advantages of ToxBLAzer™ DualScreen Kits:
|ToxBLAzer™ DualScreen Kit, 5mg, 6 to 8pH, Liquid, Fluorescent Detection Method, Cell-Permeant Permeability, ToxBLAzer™ Substrate, Chemical Substrate, Beta-Lactamase Substrate, Use with Flow Cytometer, Fluorometer, Microplate Reader|
|Protein biology, cell analysis, cellular imaging, cell-based reporter assays, cell-based and beta-lactamase assays, cell viability, proliferation and function, cell viability and cytotoxicity, drug discovery and development, target and lead identification and validation, kinase biology, kinase pathway analysis, G-protein coupled receptor biology, cell-based GPCR reporter assays, nuclear receptor biology, cell-based nuclear receptor reporter assays, pathway biology, cellular pathway analysis assays, other cellular pathway assay products, enzyme reporter assays, protein assays and analysis, beta-lactamase activity assays|
|6 to 8|
|ToxBLAzer™ DualScreen Kit|
|ToxBLAzer™ substrate, DMSO (for solution A), solution B, and solution C. ToxBLAzer™ substrate, DMSO (for solution A), solution B, and solution C should|
For Research Use Only. Not for use in diagnostic procedures.