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SERPINA1 Recombinant Human Protein, His Tag, Invitrogen™ Sino Biological™
This recombinant protein was expressed from a DNA sequence encoding the human SERPINA1 pre-protein (NP_000286.3) (Met 1-Lys 418) fused to the polyhistidine tag at the C-terminus
Marca: Invitrogen 10306H08H50
Detalles adicionales : Peso : 0.10000kg
Descripción
Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.- Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2
; the IC50 value is <3.0nM, as measured in 100μL reaction mixture containing 1.25ng trypsin, 10μM substrate, 50mM Tris, 10mM CaCl2, 0.15 M NaCl, pH 7.5 - Formulation: Lyophilized in 25mM Tris, 150mM NaCl, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
This protein is manufactured by Sino Biological Inc.
Especificaciones
| Mammalian | |
| Proteinase Inhibitor | |
| Proteins, Expression, Isolation & Analysis; Enzyme & Protein Activity Assays; Protein Assay Controls, Reference Standards & Accessories | |
| 45.7kDa | |
| 50μg | |
| <1 EU/μg | |
| Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 | |
| His Tag |
| Protease Inhibitors | |
| Assay Standard | |
| Lyophilized | |
| ≥90% by SDS-PAGE | |
| Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed. | |
| SerpinA1, PI, A1A, AAT, PI1, A1AT, mgC9222, PRO2275, mgC23330 | |
| SERPINA1 |
For Research Use Only. Not for use in diagnostic procedures.