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SELE (E-SELECTIN) Recombinant Rat Protein, His Tag, Invitrogen™ Sino Biological™
This recombinant protein was expressed from a DNA sequence encoding the rat SELE (P98105-1) extracellular domain (Met 1-Pro 494) fused to a polyhistidine tag at the C-terminus
Marca: Invitrogen 80033R08H250
Detalles adicionales : Peso : 0.10000kg
Includes: Five vials of lyophilized protein
Descripción
Selectin E (SELE), recombinant rat protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.- Activity: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells; when 5 x 104 cells/well are added to rat SELE coated plates (2μg/mL with 100μL/well), approximately 20-35% will adhere for 1 hour incubation at 37°C
- Formulation: Lyophilized in 140mM NaCl, 2.7mM KCl, 10mM Na2HPO4, 1.8mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80
- Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2mg/mL or lower; aliquot and use as soon as possible
This protein is manufactured by Sino Biological Inc.
Especificaciones
| Protein Expression and Isolation, Enzyme and Protein Activity Assay, Protein Assay Control, Reference Standards and Accessories | |
| 5 x 50μg | |
| <1 EU/μg | |
| Sele–selectin E | |
| His Tag | |
| Other Proteins | |
| Other Proteins |
| 53kDa | |
| Store at -20°C. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles. Expires one year from date of receipt when stored as instructed. | |
| SELE | |
| SELE | |
| Mammalian | |
| Lyophilized | |
| ≥90% by SDS-PAGE |
For Research Use Only. Not for use in diagnostic procedures.