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enQuireBio™ Recombinant Gilthead Seabream IGF1 Protein

A cDNA sequence encoding the IGF1 was constructed and used to recombinantly synthesize the protein.

Marca:  enQuireBio™ QP10678-10ug

Detalles adicionales : Peso : 0.01000kg

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Código de producto. 15914628

  • 103.00€ / 10 microgramos


Fecha estimada de envío
15-07-2019
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Especificaciones

Especificaciones

IGF1 Protein
10 μg
< 1.0 EU per ug protein as determined by the LAL method.
Recombinant Protein
E. coli
The protein was lyophilized from a concentrated (1 mg/ml) solution with 0.02% NaHCO3.
Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
Research Use Only
Binding assays of the 125I-Gealthead Seabream IGF1 to Gilthead Seabream or carp (Cyprinus carpio) sera resulted in high specific binding, indicating the existence of one or more IGF-binding proteins. In binding experiments to crude Gilthead Seabream brain homogenate, using human (h) IGF-I as a ligand, the respective IC50 value of hIGF1 was about fourfold lower than that of Gilthead Seabream IGF-1. Recombinant Gilthead Seabream IGF-1 exhibited mitogenic activity in a mouse mammary gland-derived MME-L1 cell line which was approximately 200-fold lower than that of hIGF1. Binding experiments to intact MME-L1 cells suggests that this difference most likely results from a correspondingly lower affinity for IGF1 receptor in these cells. In contrast, the activities of Gilthead Seabream IGF-I and hIGF-I measured by 35S uptake by gill arches from the goldfish (Carassius auratus) were identical, indicating that the recombinant Gilthead Seabream IGF-I is biologically active.
Gilthead Seabream
Untagged
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