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Rat IgG2a kappa, Super Bright 780, eBioscience™

Rat Isotype Control

Marca:  Invitrogen 78-4321-80

Detalles adicionales : Peso : 0.01000kg

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Código de producto. 15934174

  • 132.00€ / 25 microgramos


Fecha de envío estimada
del proveedor 25-11-2019
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Descripción

Descripción

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405nm; Emission: 780nm; Laser: Violet Laser

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Especificaciones

Especificaciones

Rat IgG2a kappa
Control
Super Bright 780
Liquid
Rat
Affinity chromatography
RUO
Isotype Control
Control, Flow Cytometry
0.2mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
IgG2; IgG2a; IgG2k
IgG2a, kappa
25μg
4°C, store in dark, DO NOT FREEZE!
Not Applicable
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