Thermo Scientific™ Pierce™ p53 In-Cell ELISA Kit
Measure total p53 (tumor protein 53) and tubulin using p53 and alpha-tubulin antibodies detected by fluorescence or TMB substrate in thes whole-cell microplate assays.
Marca: Thermo Scientific™ 62216
Detalles adicionales : Peso : 0.01000kg
DescripciónThe tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.
|Pierce p53 Colorimetric In-Cell ELISA Kit|
|Blocking Buffer, Components included only in the target-specific kits:Antibody #1 and Antibody #2, Janus Green Whole-Cell Stain, 50mL, 20X Tris Buffered Saline, Surfact-Amps 20, Surfact-Amps X-100, HRP Conjugate, TMB Substrate, TMB Stop Solution, Janus Green Whole-Cell Stain, Elution Buffer, Thin Plate Seal Assembly|
|96 test kit|
|1 hr 20 min|
|Multi-component kit with HRP-conjugate|
|Colorimetric Microplate Reader|
|Supernatants (10,000 cells)|
|96 microplate wells of overnight-cultured cells|