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Thermo Scientific™ Colorantes de fluoresceína y FITC y kits de etiquetado Pierce™

Activated derivatives of green-fluorescent fluorescein dye to label antibodies, proteins and other molecules for fluorescence imaging (ex 494; em 518).

Marca:  Thermo Scientific™ 46425

Detalles adicionales : Peso : 0.80000kg

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Código de producto. 11819380

  • 68.75€ / 100 miligramos


Stock EU 8
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Descripción

Descripción

Fluorescein dyes are used in wide-ranging applications including fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. FITC and NHS-fluorescein are reactive toward primary amine groups on proteins, peptides and other biomolecules, while fluorescein-5-maleimide and 5-IAF react with free sulfhydryls on cysteine residues.Most activated fluorescein derivatives are mixture of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra and for protein applications there is no need to isolate a specific isomer. The single isomer is used in high resolution separation techniques such as sequencing and capillary electrophoresis.

Highlights:

  • Amine-specific labeling: the NHS-ester or isothiocyanate efficiently labels antibodies and other purified proteins at primary amines (lysine side chains) Optimized procedure: following the standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence Single-use fluors: no need to weigh tiny amounts powder; kits contain single-use vials of reagent Efficient purification: kits include purification resin and easy-to-use spin columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery
Especificaciones

Especificaciones

Isotiocianato de fluoresceína (FITC) Pierce
Colorante fluorescente de isotiocianato de fluoresceína
Aproximadamente 50 usos para etiquetar un total de 1712 mg de IgG en condiciones normales
100 mg
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