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Thermo Scientific™ ADN polimerasa Phusion™ Hot Start II de alta fidelidad

Optimize PCR with one of the most accurate hot start DNA polymerases on the market, featuring extreme specificity and improved performance.

Marca:  Thermo Scientific™ F549S

Detalles adicionales : Peso : 0.02000kg

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Código de producto. 10441338

  • 119.00€ / 100 unidades

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Phusion Hot Start High-Fidelity DNA Polymerase is a highly accurate hot start DNA polymerase. It has now been upgraded to allow the use of even wider range of primers, including those with low melting temperatures. The new Phusion Hot Start II DNA Polymerase is ideal for PCR applications from routine PCR to highly demanding applications such as cloning and high-throughput PCR.
  • No annealing temperature limitations
  • Allows reaction setup at room temperature
  • Zero-time reactivation due to unique hot start technology
  • Fast PCR enzyme with high fidelity (over 6 times more accurate than Pfu)
  • High product yields with low enzyme amounts gives excellent cost/reaction ratio
  • Robust enzyme with high tolerance against PCR inhibitors requires minimal optimization


5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50mM MgCl2 solution. Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration

Recommended for:

Fast PCR, high-fidelity PCR, cloning, long or difficult amplicons, DHPLC, microarray



ADN polimerasa Phusion™ Hot Start II de alta fidelidad
Tampón 5x Phusion HF, tampón 5x Phusion GC, DMSO y 50 mM de solución MgCl2 Tanto el tampón de alta fidelidad (HF) Phusion como el tampón para cromatografía de gases (GC) Phusion proporcionan 1.5 mM de MgCl2 en la concentración final 1x
Temperatura de almacenamiento recomendada de - 20 °C. Estable durante seis meses desde la fecha de envasado.
PCR rápida, PCR de alta fidelidad, clonación, amplicones largos o difíciles
100 U (2 U/µl)

Current Phusion Hot Start customers can switch to Phusion Hot Start II with no changes needed to existing protocols or primers. Original Phusion Hot Start will remain available for purchase, however, all new customers are advised to purchase Phusion Hot Start II to allow more flexibility in primer design.