Invitrogen™ pHrodo™ iFL STP Ester (amine-reactive)
The amine-reactive pH-sensitive pHrodo iFL STP Ester dye is an improved version of pHrodo dye optimized for the creation of bioconjugates to be used in the study of antibody internalization, endocytosis, and phagocytosis.
Marca: Invitrogen™ P36012
Detalles adicionales : Peso : 0.00940kg
DescripciónThe amine-reactive pH-sensitive pHrodo iFL STP Ester dye is an improved version of pHrodo dye optimized for the creation of bioconjugates to be used in the study of antibody internalization, endocytosis, and phagocytosis. pHrodo iFL dye dramatically increases fluorescence as the pH of its surroundings become more acidic. It is more soluble than the original pHrodo dye, making it useful for the labeling of antibodies that may otherwise precipitate out of solution during conjugation.
• Use pH-sensitive pHrodo iFL STP Ester to make pH-sensitive bioconjugates of your choice
• Get faster, more accurate results than with any other endocytosis or phagocytosis assay—no need for wash steps or quenchers
• Multiplex with green fluorescent markers such as GFP, pHrodo iFL Green STP, and many others
The increase in fluorescence of pHrodo iFL dye as the pH changes from basic to acidic correlates with the acidification of intracellular vesicles, making it a valuable tool for the study of endocytosis or phagocytosis and their regulation by environmental factors, drugs, or pathogens. The spectral properties of pHrodo iFL dye make it useful for multi-color experiments. pHrodo iFL dye can be detected using most standard red-fluorescent filter sets and has been validated for use in a variety of applications, including flow cytometry, fluorescent microscopy, and high content screening (HCS). The lack of fluorescence of pHrodo iFL dye in a typical extracellular environment eliminates the need for wash steps or quencher dyes in the experimental workflow.
pHrodo iFL STP Ester is an amine reactive dye that can be used to make pHrodo iFL STP bioconjugates in aqueous buffer. The STP ester will react with primary amines on a protein, cell, or virus to create a stable conjugate that can be used in live cell assays or stofor later use.
Store in freezer (-5 to -30°C) and protect from light.
|pHrodo™ iFL Green STP ester, amine reactive dye|
|Store in freezer (-5 to -30°C) and protect from light.|