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Thermo Scientific™ Estándar de proteínas de rango bajo sin teñir PageRuler™

A mixture of seven purified proteins and peptides (3.4 to 100kDa) for use as size standards in peptide and small protein gel electrophoresis.

Marca:  Thermo Scientific™ 26632

Detalles adicionales : Peso : 0.01250kg

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Código de producto. 11802134

  • 76.00€ / paquete de 2


Stock EU 26
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Descripción

Descripción

The protein MW markers in this ladder resolve into clearly identifiable sharp bands when analyzed by SDS-PAGE and stained with coomassie dye or silver. The 100kDa protein band indicates the approximate position of large proteins and the 25kDa band is of greater intensity and serves as a reference band. Proteins can be detected on Western blots by staining with Ponceau S, coomassie blue or other protein stains. In addition, the ladder proteins (except for the 5 and 3.4kDa peptides) contain an integral Step-tag™ II Sequence and may be detected on Western blots using Strep-Tactin™ Conjugates. The PageRuler Unstained Broad Range Protein Ladder is supplied ready-to-use: no heating, dilution or addition of a reducing agent is required before use.

Highlights:

  • Size range – seven proteins and one peptide spanning 3.4 to 100kDa

  • Ready-to-use – supplied in a loading buffer for direct loading on gels; no need to boil

  • Sharp bands – excellent accuracy for detection in with coomassie or silver stains

  • Reference bands – 25kDa band is stronger than the others for easy orientation

  • Tagged – each protein in the ladder contains an integral Strep-tag™ II Sequence

  • Quality tested – each lot evaluated by SDS-PAGE and Western blotting

Includes:

Proteins in 62.5mM Tris-H3PO4 (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 100mM DTT, 1mM NaN3, 0.01% bromophenol blue and 33% glycerol.

Recommended for:

Accurate protein sizing on SDS-polyacrylamide gels and Western blots

Especificaciones

Especificaciones

Estándar de proteínas de bajo rango sin teñir PageRuler
8 péptidos y proteínas de 3.4 a 100 kDa en tampón de muestras de reducción
Sin teñir
Protein Molecular Weight Marker/Ladder
Visible
2 x 250 µl
De 50 a 100 carriles de minigel a de 5 a 10 ul por carril
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