Invitrogen™ Lipocalin-2 (LCN2) Mouse ELISA Kit

Descripción

The Mouse Lipocalin-2 (LCN2) ELISA quantitates Ms Lipocalin-2 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Lipocalin-2. Principle of the method The Mouse Lipocalin-2 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

El NGAL (lipocalina asociada a la gelatinasa neutrófila, lipocalina 2, siderocalina y lipocalina neutrófila) es un monómero de cadena única glicosilado de 25 kDa que forma parte de la familia de proteínas lipocalinas que se unen y transportan pequeñas moléculas lipofílicas. El NGAL es liberado por neutrófilos activados, puede formar dímeros y pequeñas cantidades de oligómeros más elevados, y forma complejos con la metaloproteinasa de matriz 9 (MMP-9, gelatinasa B). La expresión de niveles bajos de NGAL en una variedad de epitelios puede aumentar en caso de inflamación o cáncer. La expresión de NGAL en el riñón se incrementa drásticamente en caso de lesión renal isquémica o nefrotóxica.