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  5. Invitrogen™ Mouse 5'-Nucleotidase/CD73 ELISA Kit

Invitrogen™ Mouse 5'-Nucleotidase/CD73 ELISA Kit

Product Code. 17124883
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5 x 96 Tests
96 Tests
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17124883 96 Tests Pack of 96
17135629 5 x 96 Tests Each
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Product Code. 17124883 Supplier Invitrogen™ Supplier No. EM2RB

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Description

ELISA

Mouse 5'-Nucleotidase/CD73 quantitates mouse 5'-Nucleotidase/CD73 in serum, plasma, supernatant. The assay will exclusively recognize both natural and recombinant mouse 5'-Nucleotidase/CD73.

CD73 (Ecto-5-prime-nucleotidase, 5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. CD73 consists of a dimer of 2 identical 70 kDa subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. CD73 is used as a marker of lymphocyte differentiation. Consequently, a deficiency of CD73 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from CD73 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. The CD73 gene has been localized to 6q14-q21 by immunofluorescence and a study of a panel of human x mouse hybrids that contained fragments of chromosome 6 as translocations. Defects in the CD73 gene can lead to the calcification of joints and arteries, and intestinal tuberculosis. Two transcript variants encoding different isoforms of CD73 have been found.
TRUSTED_SUSTAINABILITY

Specifications

Accession Number 2210401F01Rik; 5 nucleotidase, ecto; 5 ' nucleotidase, ecto; 5 '-NT; 5 '-nucleotidase; 5 '-nucleotidase ecto; 5 '-nucleotidase, ecto (CD73); AI447961; CALJA; CD73; E5NT; Ecto-5 '-nucleotidase; eN; eNT; NT; Nt5; Nt5e; Nte; Purine 5-Prime-Nucleotidase
Assay Range 7 to 1,500 pg/mL
Assay Sensitivity 7 pg/mL
Conjugate HRP
Product Type ELISA
Sample Type Plasma, Serum, Supernatant
For Use With (Equipment) Colorimetric Microplate Reader
Gene ID (Entrez) 23959
Gene Symbol NT5E
Interassay CV <12%
Intraassay CV <10
Kit Contents Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
Label or Dye HRP
Quantity 96 Tests
Regulatory Status RUO
Sample Volume Plasma 50 μL, Serum 50 μL, Supernatant 50 μL
Storage Requirements 2°C to 8°C
Target CD73
Test Time 1 hrs 20 mins
Total Assay Time 4 hrs 45 mins
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