missing translation for 'onlineSavingsMsg'
Learn More

Invitrogen™ EdU (5-ethynyl-2’-deoxyuridine)

Measures new DNA synthesis

Marca:  Invitrogen™ E10187

Detalles adicionales : Peso : 0.00450kg

 Ver más versiones de este producto

Código de producto. 10617215

  • 1326.00€ / cada uno


En stock, listo para ser enviado: 1
Añadir a la cesta

Descripción

Descripción

EdU (5-ethynyl-2'-deoxyuridine) can be used as a replacement for BrdU (5-bromo-2'-deoxyuridine) and directly measures de novo DNA synthesis or S-phase synthesis of the cell cycle using click chemistry. Click chemistry is a method of covalently coupling an azide with an alkyne. Detection of EdU employs the copper(I) catalyzed click reaction with an azide modified fluorescent dye to form a stable triazole ring. Because of the small size of the click detection reagent, no harsh denaturation steps are needed to gain access to the DNA. Eliminating this step allows for more reproducible results, a simpler and quicker protocol and measurements which can easily be multiplexed with relevant antibody-based targets including phospho-histone H3, Ki-67, and cyclin B1 as well as dual-pulse experiments with BrdU by flow cytometry, fluorescence microscopy, microplate (HTS) or high-throughput imaging (HCS). Cell-Permeant.

Cell Analysis, Cell Cycle, Cell Proliferation, Cell Viability, Proliferation and Function, Cellular Imaging, Flow Cytometry, Flow Cytometry Cell Cycle Assays, Flow Cytometry Cell Proliferation, Flow Cytometry of Cellular Processes, High-Content Screening (HCS)

Especificaciones

Especificaciones

500mg
Solid
Room Temperature
Click-iT™
EdU
Store at -≤20°C.
Any Tissue Type, Cell Cultures, Tissue Sections, Cultured Cells, Whole Animals
Molecular Probes®
Fluorescent
Other Label(s) or Dye(s)
Videos
Documentos

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.