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MAP3K2 (MEKK2) Recombinant Human Protein
MAP3K2 (MEKK2) Recombinant Human Protein
Marca: Invitrogen PV3822
Detalles adicionales : Peso : 0.05250kg
Descripción
MAP3K2 (MEKK2) is a serine⁄threonine kinase that activates kinases involved in the MAP kinase signaling pathway.Especificaciones
| AAF63496.3 | |
| Cell Viability, Pharma and Biopharma, Cell Proliferation and Function, Protein Assays and Analysis, Target and Lead Identification and Validation, Kinase Assay | |
| 97.2kDa | |
| 10μg | |
| For research use only. Not for use in diagnostic procedures | |
| MAP3K2 (MEKK2) | |
| MAP3K2 was assayed in a two stage coupled reaction involving cascade phosphorylations of inactive MAP2K1 Wild-Type (P3093) and inactive MAPK3 (PV3312) followed by phosphorylation of myelin basic protein (MBP) substrate by activated MAPK3. The enzymes were diluted in enzyme dilution buffer before adding to the reaction. In stage one, MAP3K2 (8.33μg/mL final concentration) is incubated with inactive MAP2K1 (33μg/mL final concentration) and inactive MAPK3 (33 μg/ml final concentration) in 25mM Tris (pH 7.5), 10mM MgCl2, 0.5mM EGTA, 0.5mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100 and 100 μM ATP for 30 minutes at 30°C. In stage two, 5μL of the above reaction mix is transferred to 25μL of a reaction mix containing 25mM Tris (pH 7.5), 10mM MgCl2, 0.5mM EGTA, 0.5mM Na3VO4, 5mM β-glycerophosphate, 2.5mM DTT, 0.01% Triton™ X-100, 100 μM ATP, 500μg/mL MBP substrate and trace [ 32P]-γ-ATP and incubated for 10 minutes at 30° | |
| MAP3K2 (MEKK2) Recombinant Human Protein | |
| Human | |
| Baculovirus | |
| Serine/threonine kinase |
| 0.41mg/mL | |
| 10746, 6773 | |
| MAP3K2 (MEKK2) Recombinant Human Protein | |
| –80°C | |
| 1 Each | |
| MAP3K2 (MEKK2) Recombinant Human Protein | |
| STE11 kinase family | |
| 220nmoles of phosphate transferred to myelin basic protein (MBP) per minute per mg of total protein Activity determined at a final protein concentration of 1.39μg/mL | |
| GST Tag | |
| Kinases & Inhibitors | |
| 90% by SDS-PAGE |
For Research Use Only. Not for use in diagnostic procedures.