Epredia™ Lab Vision™ HER-2/c-erbB-2/neu (Phospho-specific) Ab-18 Mouse Monoclonal Antibody, 200μg/mL, biotinylated, BSA and azide

Descripción

c-erbB-2 (HER-2/neu) is a 185 kDa receptor tyrosine kinase belonging to the epidermal growth factor receptor family, which also includes EGFR (HER-1), c-erbB-3 (HER-3), and c-erbB-4 (HER-4). Amplification of the HER-2 gene with overexpression of the protein occurs in 20-30% of breast cancers, and tumor progression in mammary cancer is associated with elevated levels of tyrosine-phosphorylated neu and erbB-3. Tyr¹²⁴⁸ is the phosphorylation site that is most tightly linked to oncogenic transformation and coupling to the ras/MAP kinase signaling pathway. Cases of invasive breast carcinoma with the phosphorylated receptor have displayed aggressive clinicopathological features and adverse prognoses. Activation of HER-2 is frequent in ductal carcinoma in situ suggesting that HER-2 signaling plays a critical role in the early stages of breast tumorigenesis.

Host Species: Mouse

Clone: PN2A

Isotype: IgG1

Species Reactivity: Human and Rat. Others not tested.

Epitope: aa 1242-1255 with pTyr

Immunogen: Tyrosine-phosphorylated synthetic peptide corresponding to aa 1242-1255 [Lys-TAENPE-pY-LGLDVPV] from the C-terminus of human HER-2 protein (P-Tyr1248)

Molecular Weight: 185 kDa

Positive Control: EGF-treated SKBR-3 cells. About 5% of breast CA are positive for phospho-HER-2/c-erbB-2/neu oncoprotein.

Cellular Localization: Membrane

Recommended for:

• Immunohistochemistry
• Western Blotting
• Immunofluorescence
• Immunoprecipitation (Not verified)
• Flow Cytometry (Not verified)

Note:

USA: RUO; Int'l: RUO