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Thermo Scientific™ Klenow Fragment, exo-

Klenow Fragment, exo- is the large fragment of DNA polymerase I. Exhibits 5'→3' polymerase activity, but lacks the 3'→5' and 5'→3' exonuclease activities.

Marca:  Thermo Scientific™ EP0422

Detalles adicionales : Peso : 0.02000kg

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Código de producto. 10399689

  • 274.00€ / 1500 unidades


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Descripción

Descripción

Highlights

  • Lacks 3'→5' exonuclease activity
  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, fluorescein-, rhodamine-, aminoallyl-, biotin-labeled nucleotides)
  • Active in restriction enzyme, PCR, and RT buffers

Applications

  • Random-primed DNA labeling
  • Labeling by fill-in 5'-overhangs of dsDNA
  • Strand displacement amplification (SDA)
  • DNA sequencing by the Sanger method

Note

Klenow Fragment, exo- is not recommended for DNA blunting reactions prior to DNA ligation since it frequently adds one or more extra nucleotides to the 3'-terminus of blunt-end DNA substrates in a non-template directed fashion

Especificaciones

Especificaciones

5U/μL
E.coli cells with a cloned DNA fragment of the mutated polA gene
1500U
metal chelators, PPi, Pi (at high concentrations) (7)
68kDa monomer
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