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Invitrogen™ T4 Polynucleotide Kinase

Catalyzes transfer of γ-phosphate of ATP to 5′hydroxyl terminus of DNA or RNA

Marca:  Invitrogen™ 18004028

Detalles adicionales : Peso : 0.00010kg

Código de producto. 10403052

  • 510.00€ / cada uno

Fecha de envío estimada
del proveedor 03-03-2020
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  • Source: Purified from E. coli expressing the T4 polynucleotide kinase gene in a λ vector
  • Performance and Quality Testing: Ribonuclease, endodeoxyribonuclease, 3′ and 5′ exodeoxyribonuclease, and phosphatase assays; labeling efficiency tested
  • Unit Definition: One unit transfers 1nmol of phosphate of ATP to the 5′ hydroxyl termini of micrococcal nuclease-treated DNA in 30 min. at 37°C
  • Unit Reaction Conditions: 70mM Tris-HCl (pH 7.6), 0.1M KCl, 10mM MgCl2 , 1mM 2-mercaptoethanol, 0.3mM [γ-32P]ATP, 0.38mg/mL micrococcal nuclease-treated DNA, and enzyme in 150μL for 30 min. at 37°C

5′ end-labeling of DNA or RNA, Phosphorylation of synthetic linkers, Cloning, DNA & RNA Purification & Analysis, DNA Labeling, Nucleic Acid Labeling & Oligo Synthesis, Oligo Labeling, Restriction Enzyme Cloning



Store at -20°C