Invitrogen™ Human IGF2, Recombinant Protein

Descripción

IGF2 (human) Recombinant Protein, RP-8652, from Invitrogen.

Insulin-like Growth Factor II, IGF-II, is a major growth hormone made by Theca cells during gestation. While IGF-II is known to engage the IGF-I receptor (IGF1R) to mediate embryonic growth, IGF-II is also known to bind the IGF-II receptor (IGF2R). IGF2R is thought to be signaling dead receptor that acts as a sink by binding up free IGF-II. Recombinant human IGF-II is non-glycosylated protein, containing 67 amino acids, with a molecular weight of 7.5 kDa. Purity is typically greater than 97% and endotoxin level less than 1 EU/ug. The expected biological activity (ED50) is determined by the dose-dependent proliferation of FDC-P1 cells and is typically 1.5-6 ng/mL. The specific activity of this protein is 6.7x10^5 units/mg. Reconstitute using sterile water at 0.1mg/mL and centrifuge prior to opening vial. Gently pipet solution down the sides of the vial. DO NOT VORTEX sample. Store reconstituted material at -20°C and add 0.1% BSA for additional stability. Amino acid sequence: AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE.