Invitrogen™ IL-20 Human ELISA Kit

Descripción

The Human Interleukin 20 (Hu IL-20) ELISA quantitates Hu IL-20 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IL-20. Principle of the method The Human IL-20 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Las citocinas que se señalan a través de los receptores de clase II forman una familia distinta que incluye los interferones y la interleucina 10 (IL-10). La reciente identificación de varios homólogos de la IL-10 ha definido una subfamilia de citocinas que incluye a AK155, IL-19, IL-20, IL-22 e IL-24. La IL-20 pertenece a la familia IL-10 y desempeña un papel en la inflamación de la piel y el desarrollo de células hematopoyéticas. Poco se sabe sobre sus otras funciones biológicas e implicaciones clínicas. Estas citocinas muestran actividades proinflamatorias en diferentes tejidos, incluyendo la piel.