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Invitrogen™ Human IL-10 ELISA Kits

Measure levels of human interleukin-10 in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.

Marca:  Invitrogen™ EHIL102

Detalles adicionales : Peso : 0.10000kg

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Código de producto. 10443425

  • 572.00€ / 1 set


Stock EU 6
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Descripción

Descripción

Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. In humans IL-10 is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4(+) T-cell clones. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages this cytokine is a potent suppressor of the effector functions of macrophages, T-cells and NK cells. In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes. The primary structure of human IL-10 has been determined by cloning the cDNA encoding the cytokine. The corresponding protein exerts 160 amino acids with a predicted molecular mass of 18.5 kDa. Based on its primary structure, IL-10 is a member of the four a-helix bundle family of cytokines. In solution human IL-10 is a homodimer with an apparent molecular mass of 39 kDa. Although it contains an N-linked, glycosylation site, it lacks detectable carbohydrates. Recombinant protein expressed in E. coli thus retains all known biological activities. The human IL-10 gene is located on chromosome 1 and is present as a single copy in the genome. The human IL-10 exhibits strong DNA and amino acid sequence homology to the murine IL-10 and an open reading frame in the Epstein-Barr virus genome, BCRF1 which shares many of the cellular cytokine's biological activities and may therefore play a role in the host-virus interaction. The immunosuppressive properties of IL-10 suggest a possible clinical use of IL-10 in suppressing rejections of grafts after organ transplantations. IL-10 can furthermore exert strong anti-inflammatory activities.
Especificaciones

Especificaciones

P22301
Biotin
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
3586
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, HRP Dilution Buffer, 2 x 14mL
Intra-assay CV <10%; inter-assay CV <10%
2 plate kit
<3 pg/mL
50µL
No significant cross-reaction with human IL-1a, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFa, TNFß or IFNg. Approx. 2% cross-reactivity with recombinant mouse IL-10
2 x 96 microplate wells
3 hr
IL10
<10%
15.36-600 pg/mL
Human IL-10 ELISA Kit
Complete ELISA kit with coated 96-well plates
Colorimetric Microplate Reader
ELISA
Absorbance at 450nm minus absorbance at 550nm
RUO
Plasma, Serum, Supernatants, Urine
Plasma (50μL), Serum (50μL), Supernatants (50μL), Urine (50μL)
2-8°C
1 hr 20 min
Human
<10%
HRP
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