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Invitrogen™ Human IL-10 ELISA Kits

Measure levels of human interleukin-10 in human serum, plasma (EDTA, heparin, sodium citrate), urine and culture supernatant samples using 96-well plates.

Marca:  Invitrogen™ EHIL102

Detalles adicionales : Peso : 0.10000kg

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Código de producto. 10443425

  • 572.00€ / 1 set


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Descripción

Descripción

These human IL-10 ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interleukin-10 (IL-10) in samples at 50μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain pre-coated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
  • Target: Natural and recombinant human interleukin-10 (IL-10)

  • Format: Colorimetric ELISA (TMB substrate) in clear 96-well strip plate

  • Measurement: Absorbance at 450nm minus absorbance at 550nm

  • Assay Range: 15.4 to 600pg/mL

  • Sensitivity: < 3pg/mL

  • Precision: Intra-assay CV < 10%; inter-assay CV < 10%

  • Sample Types: Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)

  • Total Assay Time: 3 hours

  • Sample Size: 50μL

  • Specificity: No significant cross-reaction with human IL-1α, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFα, TNFβ or IFNγ. Approx. 2% cross-reactivity with recombinant mouse IL-10

Includes:Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.

Especificación

Especificación

P22301
Biotin
Colorimetric ELISA (TMB substrate) in clear 96-well strip plate
3586
Standard Diluent, 2 x 12mL, 30X Wash Buffer, 2 x 50mL, Adhesive Plate Covers, 12 ea, Anti-Human IL-10 Precoated 96-well Strip Plate, 2 ea, Biotinylated Antibody Reagent, 2 x 8mL, Recombinant Human IL-10 Standard, 4 vials, Stop Solution, 2 x 13mL, Streptavidin-HRP Concentrate, 2 x 75µL, TMB Substrate Solution, 2 x 13mL, HRP Dilution Buffer, 2 x 14mL
Intra-assay CV <10%; inter-assay CV <10%
2 plate kit
<3pg/mL
50µL
No significant cross-reaction with human IL-1a, IL-2, IL-3, IL-4, IL-6, IL-7, IL-8, TNFa, TNFß or IFNg. Approx. 2% cross-reactivity with recombinant mouse IL-10
2 x 96 microplate wells
3 hr.
IL10
<10%
15.4 to 600pg/mL
Human IL-10 ELISA Kit
Complete ELISA kit with coated 96-well plates
Colorimetric Microplate Reader
ELISA
Absorbance at 450nm minus absorbance at 550nm
RUO
Cell culture supernatant, urine, serum, plasma (EDTA, sodium citrate and heparin)
Plasma (50μL), Serum (50μL), Supernatants (50μL), Urine (50μL)
2-8°C
1 hr 20 min
Natural and recombinant human interleukin-10 (IL-10)
<10%
HRP
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