Invitrogen™ Endostatin Human ELISA Kit

Descripción

The Human Endostatin ELISA quantitates Hu Endostatin in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu Endostatin. Principle of the method The Human Endostatin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

COL18A1 es la cadena alfa del colágeno tipo XVIII. Este colágeno es una de las multiplexinas, proteínas de matriz extracelular que contienen múltiples dominios de triple hélice (dominios colagenosos) interrumpidos por dominios no colagenosos. El fragmento C-terminal producido proteolíticamente del colágeno tipo XVIII es la endostatina, una potente proteína antiangiogénica. Las mutaciones en este gen están asociadas con el síndrome de Knobloch. Las características principales de este síndrome implican anomalías retinianas, por lo que el colágeno tipo XVIII puede desempeñar un papel importante en la estructura retiniana y en el cierre del tubo neural.