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HLA-ABC Monoclonal Antibody (W6/32), Super Bright™ 436, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

en la oferta

Especificaciones

Antígeno HLA-ABC
Clon W6/32
Concentración 5 μL/Test
Aplicaciones Flow Cytometry
Clasificación Monoclonal
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Descripción

Descripción

Description: The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with beta 2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11-12 kDa chains. Crossreactivity is also seen in baboon, rhesus and cynomolgus monkey. Applications Reported: This W6/32 antibody has been reported for use in flow cytometric analysis. Applications Tested: This W6/32 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No. SB-4400) to minimize any non-specific polymer interactions.

HLA-ABC Monoclonal antibody specifically detects HLA-ABC in Human samples. It is validated for Flow Cytometry.
Especificaciones

Especificaciones

HLA-ABC
5 μL/Test
Monoclonal
Liquid
RUO
IgG2a κ
Affinity chromatography
Antibody
W6/32
Flow Cytometry
Super Bright 436
Mouse
Human
Primary
4° C, store in dark, DO NOT FREEZE!
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