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Thermo Scientific™ Resina HisPur™ Ni-NTA

Purifique proteínas de fusión recombinante etiquetadas con His que utilizan gránulos, columnas de centrifugación y placas de quelantes metálicos de níquel-NTA (ácido nitrilotriacético) de alta capacidad.

Marca:  Thermo Scientific™ 88221

Detalles adicionales : CAS : 64-17-5 Peso : 2.72160kg

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Código de producto. 10449164

  • 109.00€ / 10 ml


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Descripción

Descripción

The specially prepared nickel-NTA support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.

HisPur Ni-NTA Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high through-put needs. With these options, high-yield His-tag protein purification is possible at nearly any scale.

Highlights:

  • High capacity – bind up to 60mg of 6xHis-tagged protein per milliliter of resin

  • Versatile – purify proteins using native or denaturing conditions

  • Compatible – use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives

  • Flexible – available in multiple formats including bulk resin, spin columns and chromatography cartridges

  • Cost-effective – reuse the same batch of resin at least five times

  • Easy to use – pre-formulated buffers available for kit formats

Available Formats:

  • Resin Slurries – crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles
  • Spin Columns – 0.2mL (microcentrifuge), 1mL, 3mL columns
  • Purification Kits – complete kits in all three column sizes
  • Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing
  • Bulk and Custom – let us know your needs for bulk HisPur Ni-NTA Resin

Includes:

Kits contain HisPur Ni-NTA Columns

Especificación

Especificación

Resina de Ni-NTA HisPur
10 ml
Agarosa
Niquel-NTA (IMAC) sobre microesferas de agarosa entrecruzada, en suspensión en alcohol etílico al 20 %
Unión de hasta 60 mg de proteínas etiquetadas con His por ml de resina
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