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Invitrogen™ HIF-1 alpha ELISA Kit, Human

Contains sufficient reagents and buffers to assay HIF-1 alpha (hypoxia-inducible factor 1-alpha) levels in serum, plasma (EDTA, heparin, sodium citrate), and culture lysate

Marca:  Invitrogen™ EHIF1A5

Detalles adicionales : Peso : 0.01000kg

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Código de producto. 15318194

  • 1734.00€ / paquete de 5


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Descripción

Descripción

HIF1-alpha (HIF1A) is a subunit of HIF1, which is a transcription factor found in mammalian cells cultured under reduced oxygen tension. HIF-1 is a heterodimer consisting of an alpha and beta subunit, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family of transcription factors. HIF1 functions as a transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, HIF-1 activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation and tumor angiogenesis. Hypoxia which induces p53 protein accumulation, directly interacts with HIF1-alpha and reduces hypoxia-induced expression of HIF1-alpha by promoting MDM2-mediated ubiquitination and proteasomal degradation under hypoxic conditions. Recent studies suggest that induction of NOX4 by HIF1-alpha contributes to maintain ROS levels after hypoxia and hypoxia-induced proliferation. In humans, it is located on the q arm of chromosome 14. The C-terminal of HIF1A binds to p300. p300/CBP-HIF complexes participate in the induction of hypoxia-responsive genes, including VEGF. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease.
Especificación

Especificación

Q16665
Biotin
HIF-1 Alpha ELISA Kit, 480 Assays, Human Species, Immunology Research Category, Colorimetric Detection Method, Colorimetric Elisa (TMB Substrate) in Clear 96-Well Strip Plate, The Complete Kits Contain Precoated 96-Well Strip Plate(S) and Lot-Optimized Re
Solid
3091
Colorimetric Microplate Reader
ELISA
Intra-assay CV <10%; inter-assay CV <10%
1 Kit(s)
30 pg/mL
50μL
480 Assay(s)
Specific to human HIF-1A and shows cross-reactivity with mouse and rat HIF-1A
TMB
1 hr 20 min
Human
450 to 550nm
Transcription factors
HIF1A
<10%
HRP
81.92-20,000 pg/mL
Mouse and rat HIF-1A
Colorimetric ELISA (TMB substrate) in clear 96-well strip plates
Protein assays and analysis
Anti-HIF-1A precoated 96-well strip plate, lyophilized recombinant HIF-1A standard, standard diluent, 30X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, HRP dilution buffer, TMB substrate, stop solution, contains 0.16M sulfuric acid
Pre-coated 96 well plate, Anti-HIF-1A precoated 96-well strip plate, lyophilized recombinant HIF-1A standard, standard diluent, 30X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, HRP dilution buffer, TMB substrate, stop solution, contains 0.16M sulfuric acid, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
1 Kit(s)
Absorbance at 450nm minus absorbance at 550nm
RUO
Cell Lysates, Plasma, Serum
Cell Lysates (50μL), Plasma (50μL), Serum (50μL)
Human
2-8°C
480 Determinations
4 hr
480
Human
Immunology
Colorimetric
<10%
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