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Thermo Scientific™ GeneBLAzer™ NPSR1-A CHO-K1 DA Assay Kit
The GeneBLAzer™ NPSR1-A CHO-K1 DA (Division Arrested) cells contain the human Neuropeptide S Receptor 1-Isoform A stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line.
Marca: Thermo Scientific™ K1493
Descripción
The GeneBLAzer™ NPSR1-A CHO-K1 DA (Division Arrested) cells contain the human Neuropeptide S Receptor 1-Isoform A stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line. CellSensor™ NFAT-bla CHO-K1 cells (Cat. No. K1129) contain a beta-lactamase (bla) reporter gene under control of the nuclear factor of activated T-cell (NFAT) response element.
The NPSR1-A CHO-K1 DA cells and NPSR1-A-NFAT-bla CHO-K1 cells are functionally validated for Z'-factor and EC50 concentrations using Neuropeptide S. In addition, NPSR1-A-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary found in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
Especificaciones
NP_997055.1 | |
Células de división detenida | |
Fluorescente | |
NPSR1-A | |
Origen del clon unicelular | |
1 placa | |
NPSR1-A | |
CHO-K1 | |
GPCR |
Gen informador de beta lactamasa a base de células | |
Cada kit contiene cantidades suficientes de células de división detenida (DA) y sustrato para ensayar en 1 placa de 384 pocillos. (Otros materiales se solicitan por separado; consulte el protocolo). Incluye: o Células GeneBLAzer™ NPSR1-A CHO-K1 DA o Kit de carga LiveBLAzer™-FRET B/G, 70 µg o Solución D o Protocolo de ensayo GeneBLAzer™ NPSR1-A CHO-K1 o Protocolo de carga LiveBLAzer™-FRET B/G o Certificado de análisis Las células GeneBLAzer™ NPSR1-A CHO-K1 DA se envían en hielo seco y deben almacenarse en nitrógeno líquido inmediatamente después de su recepción. |
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GPCR | |
Células GeneBLAzer™ NPSR1-A CHO-K1 DA, kit de carga LiveBLAzer™-FRET B/G, 70 μg, solución D, protocolo de ensayo GeneBLAzer™ NPSR1-A CHO-K1, protocolo de carga LiveBLAzer™-FRET B/G, certificado de análisis | |
GeneBLAzer™ | |
BLA (betalactamasa) | |
Células de división detenida | |
GeneBLAzer™ |
For Research Use Only. Not for use in diagnostic procedures.