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GE Healthcare Kit de amplificación de ADN A illustra™ Single Cell GenomiPhi™

Amplifique el ADN del genoma completo a partir de una sola célula.  El kit de amplificación de ADN GE Healthcare Lifescience™ illustra™ Single Cell GenomiPhi™ combina unas capacidades exclusivas de ADN polimerasa Phi29 con una formulación optimizada para amplificar el ADN genómico a partir de 1 a 1000 células sin interferencia de fondo.

Marca:  GE Healthcare 29108039

Detalles adicionales : Peso : 0.00500kg Transport : UN number : 1814 Chem class : 8 Pack group : II

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Código de producto. 15311652

  • 1410.00€ / 100 reacciones


Fecha de envío estimada
del proveedor 04-01-2019
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Descripción

Descripción

  • For whole genome amplification of genomic DNA from as little as a single cell
  • Novel clean-up process supresses background allowing sensitivity down to 1fg of input DNA
  • Simple Isothernal strand displacement (MDA) technology using Phi29 DNA polymerase
  • Amplifications can be completed in less than 3 hours with less than 15 minutes hands-on time
  • Optimized to promote complete cell lysis with uniform denaturation of DNA prior to amplification
  • Reaction time: 2 to 4 hours
  • Product average size: >10kb
Applications

For SNP arrays, Next Gen Sequencing, aCGH

Recommended Storage

Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C


Especificación

Especificación

Single Cell GenomiPhi DNA Amplification kit combines the unique capabilities of Phi29 DNA polymerase with an optimized formulation to amplify genomic DNA from 1-1000 cells without background interference.
Genomic DNA Amplification
illustra Single Cell GenomiPhi DNA Amplification Kit
Store the kit at -70°C. The enzyme mix must be stored at -70°C; all other components may be stored at -20°C.
>10kb
illustra™ Single Cell GenomiPhi™ DNA Amplification Kit
Single Cell GenomiPhi Reaction buffer 1 x 275μL, Single Cell GenomiPhi Enzyme mix 1 x 25μL, Single Cell GenomiPhi Amplification mix 1 x 25μL, Single Cell GenomiPhi DTT Solution 1 x 100μL, Single Cell GenomiPhi Lysis buffer 1 x 100μL and Single Cell GenomiPhi Neutralisation buffer 1 x 100μL
100 Reactions
2 to 4 hours
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