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Invitrogen™ Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System

Rapid, ultrasensitive detection of β-galactosidase in reporter gene assays. Thermo Scientific Galacto-Light Plus β-Galactosidase Reporter Gene Assay System is designed for luminometers without injectors.

Marca:  Invitrogen™ T1011

Detalles adicionales : Peso : 0.00450kg

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Código de producto. 12614275

  • 656.00€ / cada uno


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Includes: Lysis solution, substrate, reaction buffer and accelerator

Descripción

Descripción

  • Wide dynamic range of β-galactosidase assay lets the user measure enzyme level accurately from femtogram to nanogram range
  • Assay sensitivity is 100- to 1000-fold better than with either isotopic/non-isotopic assays for chloramphenicol acetyl transferase (CAT) or colorimetric/fluorescent assays for β-galactosidase, providing greater sensitivity than competing assays
  • Highly sensitive assay with wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions
  • Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes
  • Assay can be completed in about one hour, providing fast assay turnaround
Sensitive
This chemiluminescent assay exhibits over three orders of magnitude greater sensitivity compared to colorimetric β-galactosidase assays and can be completed in a fraction of time required for assays for chloramphenicol acetyltransferase (CAT) and ELISA-based reporter assays.
 
Ready-to-Use
The Galacto-Light Plus system incorporates chemiluminescent substrates, reaction buffer, and light-emission accelerator containing luminescence enhancers, in a ready-to-use format. The reaction buffer is supplied at a pH that aids in discrimination of endogenous β-galactosidase, while having little effect on the bacterial form of the enzyme. This enables sensitive detection even in cell lines with relatively high levels of endogenous β-galactosidase activity.
 
Specifications
  • Assay Type: β-Gal (lacZ) Assay
  • Compatible Cells: Mammalian Cells
  • Detection Method: Chemiluminescent
  • For Use With Equipment: Luminometer (Microplate)
  • Form: Liquid
  • Format: 96-well plate
  • High Throughput Compatibility: High Throughput-Compatible
  • Readout: End Point
  • Sample Type (Specific): Cell Cultures
  • Substrate: Galacton-Plus
  • Substrate Properties: Chemical Substrate
  • Substrate Type: Beta-Gal Substrate
  • Target Enzyme: Beta-Galactosidase
Especificaciones

Especificaciones

Reporter Gene Assay System
96-well plate
Reporter quantitation⁄transfection normalization from transiently transfected mammalian cell lines as well as transfected primary cells
600 assays
Enhanced Chemiluminescence
Lysis solution, substrate, reaction buffer, and accelerator
Luminometer
Store at 2° to 8°C
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For Research Use Only. Not for use in diagnostic procedures.