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Thermo Scientific™ ARN polimerasa T7

Catalyze the 5'=>3' synthesis of RNA on either single-stranded or double-stranded DNA with T7 RNA polymerase.

Marca:  Thermo Scientific™ EP0112

Detalles adicionales : Peso : 1.51950kg

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Código de producto. 10212610

  • 133.00€ / 25000 unidades


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Descripción

Descripción

It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter.
  • Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)

Recommended for:

Synthesis of unlabeled and labeled RNA that can be used:for hybridization, in vitro RNA translation; as aRNA, siRNA, substrate in RNase protection assays, template for genomic DNA sequencing.

Note:

Consensus promoter sequences: TAATACGACTCACTATA G GGAGA. The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively.

Especificaciones

Especificaciones

20U/µL
Synthesis of unlabeled and labeled RNA that can be used:for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.
5 x 5000U
T7 RNA Polymerase
5 x 1mL of 5X Transcription Buffer
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