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Thermo Scientific™ T4 RNA Ligase (10 U/μL)

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Product Code. 10669690 Supplier Thermo Scientific™ Supplier No. EL0021
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Descripción

Catalyze the ATP-dependent formation of phosphodiester bonds between 5'-P and 3'-OH termini of oligonucleotides, single-stranded RNA and DNA.

T4 RNA Ligase catalyzes the ATP-dependent intra- and intermolecular formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxyl termini of oligonucleotides, single-stranded RNA and DNA.

The minimal substrate is a nucleoside 3',5'-biphosphate in intermolecular reaction and oligonucleotide of 8bases in intramolecular reaction.

  • Source: E. coli cells with a cloned gene 63 of bacteriophage T4
  • Molecular Weight: 43.6 kDa monomer
Quality Control:
  • The absence of ribonucleases, exodeoxyribonucleases, endodeoxyribonucleases and phosphatases confirmed by appropriate quality tests.
Source:
  • E.coli cells with a cloned gene 63 of bacteriophage T4

Molecular Weight:

  • 43.6kDa monomer

Definition of Activity Unit:

  • One unit of the enzyme catalyzes the conversion of 1nmol of 5ft.-[32P]-(A)12-18 to a phosphatase-resistant form in 30 min. at 37°C
  • Enzyme activity is assayed in the following mixture: 50mM Tris-HCl (pH 7.5), 10mM MgCl2, 10mM DTT, 1mM ATP, 10μM 5'-[32P]-(A)12-18 (10μM in 5ft.-termini)

Storage Buffer:

  • The enzyme is supplied in:20mM Tris-HCl (pH 7.5), 1mM DTT, 50mM KCl, 0.1mM EDTA, 0.03% (v/v) ELUGENT Detergent and 50% (v/v) glycerol

10X Reaction Buffer:

  • 500mM Tris-HCl (pH 7.5 at 25°C), 100mM MgCl2, 100mM DTT, 10 mM ATP

Inhibition and Inactivation:

  • Inhibitors: metal chelators, SH group-modifying reagents (8).
  • Inactivated by heating at 70°C for 10min.

Recommended for:

RNA 3'-end labeling with cytidine 3',5'-bis [alpha-32P] phosphate (1); Joining RNA to RNA (2); Synthesis of oligoribonucleotides and oligodeoxyribonucleotides (3, 4); Specific modifications of tRNAs (5); Oligodeoxyribonucleotide ligation to single-stranded cDNAs for 5ft. RACE (Rapid Amplification of cDNA Ends) (6); Site-specific generation of composite primers for PCR (7)

Note:

The recommended BSA concentration in the reaction mixture is 0.1mg/ml.

TRUSTED_SUSTAINABILITY

Especificaciones

Concentration 10 U/μL
Enzyme T4 RNA Ligase
Compatible Buffer 10X Reaction Buffer
Quantity 1,000 units
Product Type T4 RNA Ligase

For Research Use Only. Not for use in diagnostic procedures.

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