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Thermo Scientific™ Transcriptasa reversa RevertAid™

Perform efficient synthesis of full-length first strand cDNA up to 13 kb with this genetically modified M-MuLV RT for routine reverse transcription.

Brand:  Thermo Scientific™ EP0441

Additional Details : Peso : 1.51950kg

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Product Code. 10612191

  • 71.50€ / 10000 unidades


Stock EU 52
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Description

Description

The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity and a RNase H activity specific to RNA in RNA-DNA hybrids which is significantly lower than that of Avian Myeloblastosis Virus (AMV) reverse transcriptase (1, 2).
  • Efficient synthesis of full-length first strand cDNA up to 13kb
  • Optimum activity at 42°C
  • Active up to 50°C
  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)

Recommended for:

First strand cDNA synthesis for RT-PCR and real-time RT-PCR; Synthesis of cDNA for cloning and expression; Generation of labeled cDNA probes for microarrays; DNA labeling (3)

Specifications

Specifications

Síntesis de la primera cadena del ADNc para RT-PCR y RT-PCR en tiempo real, transcripción inversa a elevadas temperaturas para reducir los efectos de estructuras secundarias; síntesis del ADNc para clonación y expresión, generación de sondas de ADNc etiquetado para micromatrices
10 x 1 ml de tampón de reacción 5X
Transcriptasa reversa RevertAid
10,000U
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