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Thermo Scientific™ Maxima Probe qPCR Master Mix (2X), with separate ROX vial

Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.

Marca:  Thermo Scientific™ K0261

Detalles adicionales : Peso : 1.51950kg

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Código de producto. 11873923

  • 173.00€ / 200 reacciones

En stock, listo para ser enviado: 21

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Descripción

Descripción

Maxima Probe qPCR Master Mixes are ready-to-use solutions optimized for quantitative real-time PCR. The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added.

Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG). The use of Maxima Probe qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates.

Maxima Probe qPCR Master Mixes are compatible with most real-time thermal cyclers.

La mezcla maestra incluye Maxima Hot Start Taq DNA polymerase y dNTPs en un tampón para PCR optimizado. La solución ROX se proporciona en un vial independiente para utilizarlo en aquellas máquinas que requieran ROX como colorante de referencia pasivo. Maxima Hot Start Taq DNA polymerase en combinación con los tampones optimizados garantiza la sensibilidad y la especificidad de la PCR. La mezcla incluye dUTP para realizar un control opcional de contaminación cruzada mediante uracilo-ADN glicosilasa (UDG). El uso de Maxima Probe qPCR Master Mix en PCR en tiempo real garantiza la cuantificación específica, sensible y reproducible de plantillas de ADNc, viral, plasmídico y genómico.
  • Especificidad — la ADN polimerasa Taq Maxima Hot Start y el tampón optimizado eliminan la formación y amplificación inespecífica de los dímeros de primers.
  • Sensibilidad — detecta un número bajo de copias.
  • La solución ROX se suministra en un tubo independiente
  • Amplia gama lineal — cuantificación precisa en 9 órdenes de magnitud.
  • Universal: se puede utilizar en la mayoría de los instrumentos de PCR en tiempo real
  • Reproducibilidad y comodidad: mezcla maestra 2X lista para su uso

Recomendada para:

PCR y RT-qPCR en tiempo real con sondas de secuenciación específica en la mayoría de máquinas de PCR en tiempo real.

Especificaciones

Especificaciones

BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon
DNA, dsDNA
Primer-Probe Detection
Tube
200 reacciones
ADN polimerasa Taq
Standard
2 x 1.25 mL
Maxima
2X
Real Time PCR (qPCR)
High
qPCR
qPCR Master Mix
200 Reactions of 25 μL
Videos
SDS
Documentos

A license to perform the patented 5' Nuclease Process for research is obtained by the purchase of (i) both Authorized 5' Nuclease Core Kit and Licensed Probe, (ii) a Licensed 5' Nuclease Kit, or (iii) license rights from Applied Biosystems. This product is an Authorized 5' Nuclease Core Kit. Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155, 5,677,152, 5,773,258, 5,407,800, 5,322,770, 5,310,652. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser's own internal research. Separate purchase of a Licensed Probe would convey rights under the applicable claims of US Patents Nos. 5,538,848, 5,723,591, 5,876,930, 6,030,787, 6,258,569, 5,804,375 (claims 1-12 only), and corresponding claims outside the United States. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained from the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. Use of this product in a passive reference method is covered by the following U.S. Patent: 5,928,907 (claim numbers 12-24, 27-28) and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product in a passive reference method for the purchaser's own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser's activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Use of UDG for PCR carryover decontamination is covered by patents in certain countries and may require a license.